The Origin of Urinary β-Glucuronidase
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چکیده
منابع مشابه
Phenoxyacetohydrazide Schiff bases: β-glucuronidase inhibitors.
Phenoxyacetohydrazide Schiff base analogs 1-28 have been synthesized and their in vitro β-glucouoronidase inhibition potential studied. Compounds 1 (IC50=9.20±0.32 µM), 5 (IC50=9.47±0.16 µM), 7 (IC50=14.7±0.19 µM), 8 (IC50=15.4±1.56 µM), 11 (IC50=19.6±0.62 µM), 12 (IC50=30.7±1.49 µM), 15 (IC50=12.0±0.16 µM), 21 (IC50=13.7±0.40 µM) and 22 (IC50=22.0±0.14 µM) showed promising β-glucuronidase inhi...
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A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-D-glucuronide is cleaved to yield a chromophoric product. With the commercial E. coli enzyme, it is demonstrated that the reactions can be continuously monitored by the increase of absorbance at 405 nm. The method is highly sensitive and able to detect less than 1.4 ...
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The differentiation of renal from bladder bacteriuria is difficult on clinical grounds alone. To evaluate the correlation between site of infection and urinary beta-glucuronidase activity, 46 patients with well documented recurrent bacteriuria were studied by bilateral ureteral catheterization. Urinary beta-glucuronidase activity was also determined in 46 control subjects. In general, asymptoma...
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Since urinary steroids are excreted as conjugates, principally of sulfuric and glucuronic acids, hydrolysis is a necessary preliminary procedure which must be carried out before the steroids may be readily extracted by means of organic solvents. The effectiveness of bacterial action in the hydrolysis of conjugates of estrogens was demonstrated by Cohen and Marrian (1) who allowed putrefaction t...
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Hydrolysis of conjugates of urinary neutral reducing lipides by means of /?-glucuronidase of bacterial origin, as described in Paper I of this series (I), permits the extraction from urine of much larger amounts of these substances than may be obtained by extraction of acidified urine in separatory funnels. The isolation of a crystalline reducing steroid from normal urine which had been treated...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1965
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1965.38